Beth Adams
SOQUEL, CA
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Beth M. Adams
Contact Information:
### Old San Jose Rd., Soquel, CA, #####
Daytime Phone: (###)###-####
Evening Phone: (###)###-####
email:
Objective:
Proficient or familiar with a set of techniques pertaining to molecular biology, protein
purification, and RNA splicing. Searching for positions within biotechnology labs. Areas
of interest include HIV research and gene therapy-based research.
Availability:
From August, ####
Keywords:
Protein expression and purification, FPLC, RNA splicing, qRT-PCR
Education and Honors:
University of California Santa Cruz. ####-####
Bachelor of Science in Biochemistry and Molecular Biology, ####
Thesis: Determining the Function of the Tudor Domain of SPF## in Splicing
Awarded: Departmental thesis honors, Department of Biochemistry and
Molecular Biology
Awarded: Deans&####; award, Division of Physical and Biological Sciences
Dean’s list: Winter ####, Spring ####, and Fall ####
Golden Key Honors society: lifetime member
Graduated cum laude. GPA: #.##
Cabrillo Community College, ####-####
GPA: #.#
Alpha-Gamma-Sigma: contributing member ####-####
Student instructor for Cellular Molecular Biology through the Access program
Professional Experience:
Research Specialist, University of California Santa Cruz.
Mentor: Dr. Melissa Jurica,
MCD Biology Department, Center for Molecular Biology of RNA
###A Sinsheimer Laboratories, UC Santa Cruz, Santa Cruz, CA #####
April ####-present
Barista, Mr. Toots Coffee House.
### Esplanade, Capitola CA #####
April ####-June ####
Barista,
Espresso
Rio

###
Esplanade
Aptos
CA
#####

July
####ÂMarch
####

Barista,
###
Union
Cafe

###
Union
St.
Santa
Cruz
CA
#####

January
####ÂJuly
####

Technologies:
Protein and DNA quantification via spectroscopic analysis or absorbance: Use of Bradford reagent in
determining protein preparation yield and concentration, as well as nanodrop analysis of both DNA and
protein when appropriate.
Plasmid DNA isolation: Used to acquire DNA to determine gene identity in plasmid library as well as
determine the success and efficiency of PCR mutagenesis, followed by sequencing.
DNA gel electrophoresis: Used to analyze vector digestion and confirm gene insertion. Also used to
analyze PCR products for mutagenesis and library screening.
RNA gel electrophoresis: Used to determine the success of in vitro transcription reactions.
Protein production: E. coli based protocols, including transformation, inoculation, induction, harvest, and
purification of protein. Produced and purified milligram amounts of protein for downstream applications.
Also used wheat-based cell free systems for proof of concept project.
SDS-PAGE: Post protein production purity gels allow tracking of production and purification, as well as
fraction identification for FPLC purified proteins.
Column chromatography: Gravity columns including IMAC and amylose resins used in affinity
purification. Ion exchange and size exclusion columns using an AKTA FPLC.
High throughput PCR screening (use of ## well format, qRT-PCR): Used to determine the effects of
adding various concentrations of exogenous protein to in vitro splicing reactions. Analyze splicing
efficiency by determining mRNA quantity.
Site directed PCR mutagenesis (including primer design): Created point mutations within specific protein
domains in order to explore the functions of those domains in the context of RNA splicing.
RNA substrate transcription: Both cold and radioactive substrate to allow for the downstream
quantification of spliced mRNA under given conditions.
References Available Upon Request